Part:BBa_K112800:Design

{mRFP} Red Fluorescent Protein CDS in BBb standard

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 555
  Illegal AgeI site found at 667
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

PCR ca8373/ca8374 on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
-----------------------------------------------
ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc